Table 5. Amplification and sequencing primers for KIR3DL1/S1 typing.

Name	Specificity	Primer Sequence (5′ 3′)	Location	Annealing Temperature
L1S1-1FPCR	5′ Exon 1	CGAGGTGTCAATTCTAGTGAGAG	Intron 1	61 to 51°Cc
L1S1-1RPCR	3′ Exon 1	CACTTCAGGCCCATAACTCCAC	Intron 2	61 to 51°Cc
L1S1-1FSEQ	Exon 1	CGAGGTGTCAATTCTAGTGAGAG	Intron 1	53.0°C
L1S1-1RSEQ	Exon 1	CTAGGCCCATATCTTTACCTCC	Intron 2	55.0°C
L1S1-2-3FPCR	5′ Exon 2	GAGATCCTTGTTCCTGGGG	Intron 2	56.2°C
L1S1-2-3RPCR	3′ Exon 3	CGTCTCCCTCCCACTACAC	Intron 3	56.2°C
L1S1-2FSEQ	Exon 2	CAGCGAGGGTGAGTTTAC	Intron 2	56.9°C
L1S1-2RSEQ	Exon 2	GAGGGTCCCCTCTTCCTAGTG	Intron 3	55.0°C
L1S1-3FSEQ	Exon 3	GTGGAAATGGGGAGAATCTTCTGG	Intron 3	55.0°C
L1S1-3RSEQ	Exon 3	CAGAAGCTCTGGGATTCAG	Intron 4	58.0°C
L1S1-4FPCR	5′ Exon 4	CATGCAGCCTGTCCTCTTC	Intron 4	57.6°C
L1S1-4RPCR	3′ Exon 4	ACACGGCATCTGTAGGTGG	Intron 5	57.6°C
L1S1-4FSEQ	Exon 4	GGGAGGAGAGAGACAGACACG	Intron 4	53.0°C
L1S1-4RSEQ	Exon 4	CAGACCTCACCAAGTCAC	Intron 5	52.0°C
L1S1-5FPCR	5′ Exon 5	GACAGAGAGGCAGACAGAGAGG	Intron 5	64 to 54°Cc
L1S1-5RPCR	3′ Exon 5	CTGACTCCGCCCTCACACCTG	Intron 6	64 to 54°Cc
L1S1-5FSEQ	Exon 5	GAGAGAGAGAGAGAGAGCATTAG	Intron 5	55.0°C
L1S1-5RSEQ	Exon 5	CTCTGCATCTGTCCATGCTTTTC	Intron 6	55.0°C
L1S1-7-9FPCRa	5′ Exon 7	GCTATAACTGAGAAAGCAGGAGG	Intron 7	64 to 54°Cc
L1S1-7-9RPCRa	3′ Exon 9	CATTTGTAAGCAAGWGAGAGGCAC	Intron 9	64 to 54°Cc
L1S1-7FSEQa	Exon 7	GGGTGCTTGTCCKAAAGAGAYGC	Intron 7	58.9°C
L1-9FSEQa , b	Exon 9	CACTCAGCATTTCCCTCCCTCAC	Intron 9	54.4°C
L1-9RSEQa , b	Exon 9	GGCTGTTGTCTCCCTAGAAGACG	Intron 10	58.0°C

^aDoes not amplify 3DL1*059, 3DL1*060, 3DL1*061.

^b3DL1 only.

^cTouchdown PCR temperature range.