. 2011 Dec 28;6(12):e29210. doi: 10.1371/journal.pone.0029210

Table 2. Na⁺ transport kinetic constants for NHE1 in Amphiuma RBCs stimulated to steady-state in isosmotic solution with 500 nM CLA, or mildly hyperosmotic (1.2×IR) solution ± CLA.

Treatment	Km(mM Na+)	Jmax (mmoles Na+/Kg dcs minute
Isosmotic (1×IR)	NA	0.02±0.01^*
Isosmotic (1×IR) + CLA	44.3±7.7	10.4±1.4
Hyperosmotic (1.2×IR)	43.3±36.8	3.4±1.4
Hyperosmotic (1.2×IR) + CLA	44.4±11.8	19.8±2.5

Kinetic constants are expressed as K_m or J_max ± SEM. Values for K_m are similar in all 3 conditions, while J_max is increased by CLA treatment relative to hyperosmotic medium alone. Estimated* (by hyperbolic curve-fit) J_max activity in the unstimulated isosmotic condition is also shown.

Table 2. Na+ transport kinetic constants for NHE1 in Amphiuma RBCs stimulated to steady-state in isosmotic solution with 500 nM CLA, or mildly hyperosmotic (1.2×IR) solution ± CLA.

Table 2. Na⁺ transport kinetic constants for NHE1 in Amphiuma RBCs stimulated to steady-state in isosmotic solution with 500 nM CLA, or mildly hyperosmotic (1.2×IR) solution ± CLA.