Figure 1. Body weight and fat content of WT and GPR82-KO mice.

(A) Body weight in 15-week-old mice under standard diet and Western diet. Body weights of animals under standard diet (male: WT n = 26, KO n = 30; female: WT n = 10, KO n = 7) showed a significant difference between WT (open bars) and KO mice (filled bars). Animals under Western diet (male: WT n = 26, KO n = 28; female: WT n = 24, KO n = 22) developed higher body weights and the weight differences between male WT and KO mice remained significant. (B). Body fat content of mice under standard (male: WT n = 26, KO n = 30; female: WT n = 10, KO n = 7) and Western diet (male: WT/KO n = 10; female: WT/KO n = 9) determined by QMR. The body fat content of KO mice was significantly reduced compared to WT animals under standard and Western diet. (C) Differences in visceral fat content between 3-month-old female WT and KO mice were already macroscopically found under standard diet. (D) The average size of adipocytes in perigonadal fat of male KO mice was smaller than in WT kept under Western diet and, therefore, the adipocyte number per mm² was higher in KO. Data are given as mean ± SD. The body weight development of WT and KO mice under standard and Western diet from 4 to 14 weeks is given in Figure S5. *P<0.05, **P<0.01, ***P<0.001.