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. 2011 Dec 28;6(12):e29600. doi: 10.1371/journal.pone.0029600

Figure 3. Effect of TFA on endothelial superoxide production.

Figure 3

Human endothelial cells were treated with either 100 µM of palmitate, linoleic acid, transvaccenic (trans-C18:1 (11 trans)), linoelaidic (trans-C18:2 (9 trans, 12 trans)), and elaidic (trans-C18:1 (9 trans)) for 3 h. A. Superoxide levels were measured by ESR and the spin trap CMH. Fold increase over the control condition (BSA alone) was calculated. (n = 3, *p<0.05, compared to BSA control). B. Cells were pretreated with either of two inhibitors of superoxide (SOD, 100 Units/ml or DPI 25 µM) prior to stimulation with Elaidic or Linoelaidic acid. IL-6 levels as measured by ELISA.