Figure 6. The truncated ZFYVE27^ΔHR3 impairs the ability of wild-type ZFYVE27 to promote neurites in primary neuronal cells.

(A) Control primary neurons stained for β-III tubulin, a neuronal marker. Overexpression of E2-ZFYVE27^WT (B) and E2-ZFYVE27^ΔHR3 (C) in primary neuronal cells. Arrow in (C) showing the swelling of the cell soma of a neuronal cell expressing E2-ZFYVE27^ΔHR3. (D–F) Overexpression of both wild-type ZFYVE27 (c-Myc-ZFYVE27^WT and E2-ZFYVE27^WT) proteins revealed an enhanced neurites formation from the cell soma and showed co-localization of ZFYVE27 monomers. (G–I) Co-expression of both the c-Myc-ZFYVE27^WT and mutant E2-ZFYVE27^ΔHR3 in primary neurons showed the co-localization of both forms of ZFYVE27 mostly in cell soma and the neuron failed to produce normal length axon as well as /dendrites. Scale bars – 20 µm (A–C), 50 µm (D–F), 10 µm (G–I).