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. 2011 Dec 28;6(12):e29486. doi: 10.1371/journal.pone.0029486

Figure 2. Fluorescent eye marker visibility and variability.

Figure 2

(A) Fluorescent proteins expressed in the lens can be visualized under a handheld flashlight. All five fluorescent eye markers can be seen using the GFP flashlight while only mOrange, tdTomato, and mPlum can be seen using the RFP flashlight. (B) EGFP and tdTomato marker expression varies between transgenic lines (Low, Medium, High). Inset shows EGFP “high” line is visible in room light with no excitation. EGFP “medium” and tdTomato “high” transgenic lines are also shown in pigmented (Agouti, Black) backgrounds. Note that the visibility of fluorescent eye markers is reduced in pigmented backgrounds.