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. 2011 Dec 28;6(12):e29486. doi: 10.1371/journal.pone.0029486

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Cornett et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) tdTomato fluorescent eye marker co-segregates with and accurately marks the presence of an unrelated transgene (Luc-PB[mut]7). One litter of pups numbered 1-7 is shown. The unrelated transgene was amplified by PCR (upper panel) and the tdTomato marker was visualized under the GFP flashlight in room light (lower panel). (B) Schematic drawing of PCR strategy. One PCR primer (small arrows) was designed against the 3′ end of the Luc-PB[mut]7 transgene (gray box) and the other primer against the αA-crystallin promoter (white box) driving the tdTomato marker transgene. (C) The hybrid PCR product from the transgene concatamer is present in two transgenic animals (+) but not wildtype littermates (−).