Figure 4. Impaired IPCC, but not islet, graft survival in syngeneic recipient mice.

Islets freshly isolated from B6 donors or IPCCs that differentiated from B6-derived ASCs were transplanted under kidney capsule of either B6.Rag-/- or WT B6 recipient mice that were rendered diabetic by a single injection of streptozotocin before transplantation. Islet and IPCC syngraft rejection was observed (A). In separate groups, transplanted islets or IPCCs were retrieved and dissociated with trypsin-EDTA one week after transplantation. Cells were then stained with PE-conjugated PDX-1 and their apoptosis was measured by a TUNEL method (B). Histograms are gated on PDX-1-positive β cell population. One representative of three separate experiments is shown.