Figure 3. cyp6a17 is expressed in the mushroom bodies.

(A–E) cyp6a17 reporter expression by cyp6a17^MB-Gal4>UAS-mCD8:GFP (green). (A) cyp6a17 was strongly expressed in the αβ lobe (white and open triangle) and γ lobe (white triangle) of the mushroom bodies. (B) The GFP expression was also detected in the ellipsoid bodies (EB, white arrowhead) and pars intercerebralis (PI, white and open arrowhead). (C) cyp6a17^MB-Gal4 derived GFP was expressed in the Kenyon cells (Kc) and Calyx (Ca) of the mushroom bodies. (D) A cross section of the peduncle shows GFP staining along the outer rim that may correspond to the γ lobe (red in Fig. 3E). It also shows weak circular staining in three ring structures that appear to be αβ lobes. Note that there is no staining inside the rings that may correspond to the αβ core. (E) Schematics of Figure (D). Red may correspond to axon fibers for γ lobe; blue for α′β′; gray for αβ; yellow for αβ core based on their characteristic position in the peduncle. (F) Rescue of cyp6a17 mutant phenotypes by cyp6a17^MB-Gal4>UAS-cyp6a17. Temperature preference profile and avoidance index (AI) of the mutant line expressing the cyp6a17 transgene (cyp6a17^Δ173; cyp6a17^MB-Gal4>UAS-cyp6a17) are shown. Black bars, AI_High; gray bars, AI_Low. Two asterisks, P<0.001.