TABLE 2.

ELISPOT Assay: Comparison of Results on Same Samples Tested in 2005 (Prior Study) and 2009 (Current Study)

		Beef IFA		Vegetable IFA*
		Assay (2005)†	Assay (2009)†	Current Study
Patient			(Number of spots)‡
Expt. 1:	1	436	381	1
	2	8	0	0
	3	99	69	0
	4	567	442	0
	5	0	0	0
Expt. 2:	6	436	207	2
				0
				3
				17
				3
				0
				2

^*Samples run in same assay on current patients who received the gp100:209-217 (210M) peptide in vegetable IFA.

^†Results of assay on aliquots from the same samples tested in 2005 and in 2009.

^‡gp100:209-217 spots minus control.