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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Microbes Infect. 2011 Aug 30;14(1):43–49. doi: 10.1016/j.micinf.2011.08.003

Fig. 5.

Fig. 5

Chlamydia pneumoniae does not co-localize with the SREC-I receptor. Endothelial cells were inoculated with C. pneumoniae at 4°C for 30 min to permit attachment, but not internalization. Following fixation of cells, double immunofluorescence labeling of infected cells was done by staining the SREC-I receptor by incubating with anti-SREC-I antibody followed by rabbit anti-goat IgG conjugated to Alexa Fluor 647 and C. pneumoniae with FITC conjugated monoclonal antibody CF2 (green fluorescence). As a negative control, infected cells were stained by incubating with anti-SRA antibody followed by rabbit anti-mouse IgG conjugated to Alexa Fluor 549 and C. pneumoniae with FITC conjugated monoclonal antibody CF2 (green fluorescence). Co-localization was determined by confocal fluorescence microscopy using a LSM 510 Zeiss confocal microscope. Panels A and D - Staining of C. pneumoniae with the genus specific FITC conjugated CF2 antibody. Panel B - Staining of endothelial cells with anti-SREC-1 receptor antibody. Panel C - Panels A and B are superimposed demonstrating that C. pneumoniae does not co-localize with the SREC-I (SR-F) receptor. Panel E - Absence of staining of endothelial cells with anti-SR-A receptor antibody. Panel F - Panels D and E are superimposed. Arrows highlight stained organism/cells.