Table 3.
SPR responses of the immunosensor incorporating Anti-His IgG F(ab’) fragment towards rSPI-His6 in PBS buffer pH 7.4 (137 mM NaCl, 10 mM phosphate, 2.7 mM KCl) and in the presence of Pichia pastoris culture medium. The control sample: protein rSPI2 in PBS and medium alone.
| Analyte | Response |
|---|---|
| rSPI2-His6 in PBS | y = 36.40 log(x) + 183.20 r2 = 0.987, σ = 2.3 |
| rSPI2 in PBS | y = 1.92 log(x) + 13.54 r2 = 0.834, σ = 1.9 |
| rSPI2-His6 in culture medium | y = 32.28 log(x) + 145.29 r2 = 0.979, σ = 2.4 |
| Culture medium without rSPI2 or rSPI2-His6 | y = 3.05 log(x) + 10.74 r2= 0.963, σ = 2.0 |
y = SPR response (milidegrees); x = analyte concentration; 10 pg/mL < X < 1ng/mL rSPI2-His6; r2—correlation coefficient; σ—standard deviation, n=9