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. 2010 Jun 21;10(6):6172–6194. doi: 10.3390/s100606172

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© 2010 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 2. — Detection of SirT1 mRNA and telomerase activity in rat RSCs.(A) The amount of SirT1 mRNA was detected by Q-PCR from RSCs of SD rats of different ages, and. *p < 0.05; **p < 0.01 versus 2- and 4-month-old RSC groups. Data shown here are the means ±SD of three independent experiments. (B) Telomerase activities were quantified using a telomerase repeat amplification protocol (TRAP) assay. RSCs from rats in different ages were lysed in CHAPS lysis buffer, and equal protein quantities (300 ng) were subjected to TRAP analysis to determine telomerase activity. Negative control: without cell lysate; positive control: H1299 cell lysate.