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. 2010 Jun 21;10(6):6172–6194. doi: 10.3390/s100606172

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© 2010 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — Detection of SirT1 mRNA in RSC isolated from the posterior ciliary margin of human donor. (A) The SirT1 mRNA expression level detected by qPCR in RSCs from adult groups (n = 19) was significantly decreased compared to that in RSCs from juvenile group with donor age younger than 10 years old (n = 2, **p < 0.01). (B) The regression plot showed that the SirT1 level was negatively correlated with age in RSCs. (C) Human samples were divided into three groups based on age. The bar chart demonstrated that the SirT1 mRNA level was significantly decreased with age. (D) Neurospheres formation showed increased in patients younger than 50 years old (left upper) than those older than 65 years old (left lower). Bar: 100 μm. Data shown here are the mean ± SD of three experiments.