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. 2010 Jun 21;10(6):6172–6194. doi: 10.3390/s100606172

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© 2010 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 5. — Resveratrol (RV) increase cell viability in RSCs via the activation of SirT1. (A) RSCs from 12-month-old rats were treated with 0, 5, 10, 15, and 20 μM of RV for 48 hours. *p < 0.05: 5 μM RV versus control. **p < 0.01: 10, 15 and 20 μM RV versus control. (B) Cell viability for 48 hours were shown in RSCs treated with 0, 5, 10, 15, and 20 μM of RV. (*p < 0.05: 15 μM RV versus control). (C) Western blots showed SirT1 expression levels in RSCs transduced with lentivirus carrying luciferase shRNA (sh-Luc; vector control), and SirT1 shRNA (sh-SirT1). (D) Cell viability for 48 hours were shown in RSCs treated with 0 or 15 μM of RV after knock down by sh-Luc or sh-SirT1 (*p < 0.05: 15 μM RV versus control; *p < 0.05: 15 μM RV versus 15 μM RV+ sh-SirT1). Data shown here are the mean ±SD of three experiments.