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. 2011 Nov 25;12:582. doi: 10.1186/1471-2164-12-582

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Copyright ©2011 Rangel-Salazar et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Methylation analysis of VLR-regulated genes. A and B, COBRA and bisulfite-modified DNA sequence analysis, respectively. A, values on the y-axis are 2^-ΔCPin comparison with the average of mock digestion triplicates. An increase in methylation results in relatively low 2^-ΔCPvalues. Black bars, control; white bars, VLR-stimulated. Bars, standard deviations. B, numbers on the vertical axis indicate the position of the analyzed sequence relative to the transcription start site (bp). Ticks represent CpG dinucleotides. Horizontal bars represent the extent of methylation of the corresponding residues in the clones analyzed.