Table 1.
TF speciesa | Source | Calculatedb | MALDI-TOF | Differencec | SDS-PAGE |
---|---|---|---|---|---|
rTF1–243 | E.Coli | 27,423 | 27,800 | 377 | 31,000 |
rTF1–243”D” | E.Coli | 27,423 | 27,800 | 377 | 31,000 |
rTF1–263G | Sf9 | 29,592 | 33,196 | 3,604 | 37,000 |
rTF1–263D | Sf9 | 29,592 | 30,202 | 610 | 33,000 |
pTFG | Human placenta | 29,592 | 36,179 | 6,605 | 45,000 |
pTFD | Human placenta | 29,592 | ND | ND | 34,000 |
p-placental, r-recombinant, G-glycosylated, D-deglycosylated
calculated from amino acid composition;
difference between calculated mass by amino acid composition and that obtained by MALDI-TOF.
From J Biol Chem 2010;285:3371–82 (26).