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. 2011 Nov 4;286(52):44646–44658. doi: 10.1074/jbc.M111.265462

FIGURE 1.

FIGURE 1.

PKC is upstream of PKA in Ang II-induced RGS2 mRNA accumulation in rat aortic VSMC. A, pretreatment (30 min) of cells with various concentrations of GF109203X inhibited Ang II (100 nm, 1 h)-induced RGS2 mRNA accumulation in a concentration-dependent manner. B, pretreatment of cells with various concentrations of H-89 for 30 min inhibited Ang II-induced RGS2 mRNA accumulation in a concentration-dependent manner. C, pretreatment of cells with GF109203X (3 μm, 30 min) inhibited PMA- (100 nm, 1 h) but not FSK- (10 μm, 1 h) induced RGS2 mRNA accumulation. D, pretreatment of cells with H-89 (20 μm, 30 min) inhibited both PMA- and FSK-induced RGS2 mRNA accumulation. Veh, vehicle (Me2SO2). Data are represented as mean ± S.E. of at least three independent experiments. **, p < 0.01; ***, p < 0.001 versus basal. NS, not significant; †, p < 0.05; ††; p < 0.01; †††, p < 0.001 versus Ang II stimulation in (A and B), and PMA or FSK stimulation (Veh) in C and D.