FIGURE 2.

iPLA₂β links PKC and PKA in Ang II-induced RGS2 mRNA accumulation in rat aortic VSMC. A, pretreatment of cells with BEL (1 μm, 30 min) inhibited PMA- but not FSK-induced RGS2 mRNA up-regulation. B and C, suppression of iPLA₂β expression with iPLA₂β antisense oligodeoxynucleotides but not sense oligodeoxynucleotides, as described (16), inhibited PMA- but not FSK-induced RGS2 mRNA accumulation. D and E, cells were pretreated (30 min) with GF (3 μm) or H-89 (20 μm) and then stimulated with Ang II (100 nm, 1 h) in the presence or absence of 30 μm AA and LPC as described (16). Data are represented as mean ± S.E. of at least three independent experiments. ***, p < 0.001 versus basal. ††, p < 0.01; †††, p < 0.001; nonsignificant (NS) versus PMA or FSK stimulation (Veh) in A-C, and Ang II stimulation in D and E.