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. 2011 Nov 4;286(52):45041–45047. doi: 10.1074/jbc.M111.260737

FIGURE 5.

FIGURE 5.

Inhibition of PKA activity is involved in the effect of albumin on the sodium pump. LLC-PK1 cells were grown in 6-well plates and incubated for 30 min (A) or overnight (B) in medium depleted of serum in the absence or in the presence of 0.01 mg/ml albumin. After treatment, the cells were washed with PBS2+, and PKA or (Na+ + K+)-ATPase activities were measured. A, modulation of the effect of albumin on the PKA activity by 10 pm PMA, 0.1 μm wortmannin, or 0.1 μm calphostin C (n = 7). B, role of PKA in modulation of the sodium pump by albumin. 10 pm PMA, 0.1 μm wortmannin, 0.1 μm calphostin C, 1 μm Bt2cAMP, or 1 μm forskolin was added when indicated (n = 5). *, statistically significant in relation to the control (in the absence of albumin) or to #, 0.01 mg/ml albumin (p < 0.05).