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Phylogenetic analysis of cyclotide precursors in the Hedyotis genus. The tree was constructed using eight cyclotides precursors: hedyotide B1 and B2 from H. biflora; kalata B1, B2, B3/6, and B7 from O. affinis; hcf-1 from H. centranthoides; and cycloviolacin O8 from V. odorata. Cycloviolacin O8 was used as an outgroup to root the phylogenetic tree.
