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. 2011 Nov 1;286(52):44937–44944. doi: 10.1074/jbc.M111.309351

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Incorporation of gaseous ¹³CO into the RH of R. eutropha. A, IR spectroscopic analysis of RH purified from cells grown heterotrophically on fructose and glycerol at varying concentrations of gaseous ¹³CO added to the head space. The spectra were normalized on the CN stretchings. B, the peak height ratio (¹³CO peak height compared with the sum of ¹²CO and ¹³CO peak heights) as a function of ¹³CO concentration in the headspace, was fitted with a logistic, sigmoidal function: y(x₀) = (A_i-A_f/1+(x/x₀)^ρ)+A_f. Fitting parameters: A_i (initial y value) = 0; A_f (final y value) = 100; x₀ center value: y(x₀) = (A_i+A_f)/2) = 13.0186 and ρ (rate) = 1.0135.