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. 2011 Nov 8;286(52):45156–45164. doi: 10.1074/jbc.M111.297275

FIGURE 5.

FIGURE 5.

rAb-53 binding to AQP4 in vivo following intracerebral injection in mouse brain. A, spatial distribution of rAb-53-Cy3 (red) in brain at 3 h after injection. AQP4 stained green. White line, needle track; yellow dashed line, area of rAb-53-Cy3 diffusion; white square, area where micrographs in B and C were obtained. B, confocal fluorescence microscopy showing perivascular colocalization of rAb-53-Cy3 (red) with AQP4 (green) at 3 h after injection in wild-type mice (+/+). Controls included injection of (non-NMO) IgG in wild-type mice and rAb-53-Cy3 in AQP4-null mice (−/−). C, localization of rAb-53-Cy3 (red) at 20 min and 1, 3, and 24 h after injection, showing perivascular localization with AQP4 (green). Micrographs are representative of studies done on 3–4 mice at each time point.