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Determination of the binding stoichiometry between HPX-1 and the α1(I)772–787 THP by sedimentation equilibrium AUC. The horizontal dashed lines indicate the theoretical Mr for the free monomeric HPX-1 (23.6 kDa) and a 1:1 complex of HPX-1 with the α1(I)772–787 THP (34.5 kDa). The error in apparent molecular mass (2.1 kDa) for each point is estimated as the S.D. of the apparent molecular mass from the AUC studies on the free HPX-1 (see supplemental Fig. S1).
