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Mutant httEx1 ROS induction is poly(Q) length-dependent and occurs due to an expanded poly(Q) stretch only. A, DCF oxidation in HeLa cells expressing httEx1Q47-Q72 or -Q97mRFP compared with httEx1Q25mRFP (set at 1). B, ROS production due to expression of poly(Q)81-GFP constructs in HeLa cells at 24 h equivalent to that seen for httEx1Q97EGFP. For A and B, means ± S.E., n = 4 independent experiments, Student's t test (unpaired), *, p < 0.05.
