FIGURE 6.

In vitro poly(Q) aggregation is associated with hydrogen peroxide (H₂O₂) formation that is inhibited by the single chain antibody MW7. A, H₂O₂ production due to incubation of httEx1Q20/Q53. httEx1Q53 was also incubated in the presence of catalase, n = 6 independent experiments. B, addition of MW7 (1:1 molar ratio) prior to cleavage of GST from httEx1Q53 and reduction of oligomer size (5 h, AFM height measurements), n = 3 independent experiments. C, H₂O₂ production after 5 h of httEx1Q20/Q53 incubation with/without MW7 as in B, n = 4 independent experiments, means ± S.E. (error bars). Student's t test (unpaired), *, p < 0.05; **, p < 0.01; ***, p < 0.001.