TABLE 1.

Rate and affinity constants for binding of pTf by TbpA and TbpB

The rate and dissociation constants were calculated from the recorded sensorgrams for the binding of TbpA and TbpB to immobilized pTf on SPR biosensor. polyhis, polyhistidine; aa, amino acids.

Protein	kon	koff	Kd
	m−1s−1	s−1
ApH49 TbpB (aa 2–528)	5.21 × 105	7.97 × 10−3	15.3 ± 0.1 nm
ApH49 TbpB (aa 37–528)	3.75 × 105	6.87 × 10−3	18.3 ± 0.5 nm
ApH49 polyhisTbpB (aa 37–528)	1.75 × 105	7.7 × 10−3	44 ± 1 nm
ApH49 polyhisTbpA	5.96 × 104	7.84 × 10−6	0.13 ± 0.04 nm