TABLE 1.

Uptake of folic acid by Xenopus oocytes expressing PfFT1 or PfFT2

Shown is [³H]folic acid uptake over 40 min by oocytes injected with water or with cRNA for either of the PfFTs. Data are the means and S.D. Experimental groups contained 27–48 oocytes per group. Data were obtained from at least three independent experiments (n = 3). Statistical analysis was carried out with Mann-Whitney U tests between groups of oocytes injected with PfFT1 or PfFT2 (rows) and between groups of oocytes incubated with different radiolabeled substrates (columns). NS (not significant) denotes p > 0.05.

Substrate	Uptake of folic acid			p values
	Water control	PfFT1	PfFT2
	pmol/oocyte/h
30 nm [3H]folic acid	1.07±0.27	2.92±0.48	3.45±0.71	<0.001
30 nm [3H]folic acid + 200 μm folic acid	0.42±0.16	1.21±0.39	1.07±0.27	<0.01
30 nm [3H]folic acid + 200 μmpABA	0.91±0.31	1.32±0.41	0.85±0.25	NS
p values	NS	<0.001	<0.001