TABLE 1.

Apparent affinities of recombinant Pyd PDZ2 for different PtdInsP species as determined by equilibrium SPR analysis of 5% PtdInsPs in the background of DOPC liposomes or composite liposomes (30% PC, 20% PS, 40% PE, 5% PtdIns)

	KDapp
	PtdIns3P, PtdIns4P, PtdIns5P	PtdIns(3,4)P2	PtdIns(3,5)P2	PtdIns(4,5)P2	PtdIns(3,4,5)P3
	μm
DOPC liposomes	>30	18 ± 2	9.5 ± 0.6	15 ± 5	2.2 ± 0.2
Composite liposomes	NDa	ND	ND	0.7 ± 0.1	0.7 ± 0.2

^a ND, not determined.