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. 2011 Nov 3;286(52):44441–44448. doi: 10.1074/jbc.M111.237115

FIGURE 1.

FIGURE 1.

Down-regulation of ATG7 by depletion of TDP-43. A, upper panels, semi-quantitative RT-PCR analysis of the total RNAs from Neuro2A cells transfected with scRNA oligonucleotide (lane 1) or siRNA-1 oligonucleotide (lane 2). PCR primers specific for the Atg7 mRNA and GAPDH mRNA were used in PCR. Note the down-regulation of the level of Atg7 mRNA in siRNA-1 oligonucleotide-treated cells, as quantitatively presented in the histogram on the right. ***, p value < 0.001. Lower panels, the data of Western blotting analysis of total lysates from Neuro2A cells transfected with the scRNA oligonucleotide (lane 1) and siRNA-1 oligonucleotide (lane 2), respectively. Note the decreases of amounts of both the TDP-43 and ATG7 proteins in lane 2. ***, p < 0.001. The decrease of the ATG7 protein upon siRNA-1 treatment is also quantitatively presented in the histogram. B, repeat of the experiments in A above with use of the siRNA-2 oligonucleotide. Note the decreased amounts of both the Atg7 mRNA (left panel, lane 2) and the ATG7 protein (right panel, lane 2). C, decrease of ATG7 expression in NSC34 and NIH3T3 cells upon TDP-43 knockdown by siRNA-1. The levels of Atg7 mRNA in NSC34 and NIH3T3 cells were assayed by RT-PCR after transfection of the cells with siRNA-1 oligonucleotide for 48 h. Note the decrease of the amounts of Atg7 mRNA (left panel, lanes 2 and 4) upon knockdown of the TDP-43 protein by si-RNA-1 (right panel, lanes 2 and 4).