FIGURE 6.

Inhibition of cisplatin-dependent apoptosis in HeLa cells by CARD-derived peptides. HeLa cells were treated with a Lipofectamine/peptide (100 μm) mixture for 4 h, followed by administration of 20 μm CDDP. Cells were harvested after 24 h, and cytosolic extracts were supplemented with caspase assay buffer containing 20 μm Ac-DEVD-afc substrate for caspase-3 activity evaluation. Data correspond to the mean ± S.D. (error bars) of three independent experiments and represent the inhibition percentages obtained with reference to a control sample incubated in the absence of peptides.