TABLE 3.

Biological activity of synthetic peptides derived from Apaf-1 CARD phage display

Peptide	Sequence	IC50a	IC50b
		μm	μm
Phg1	WPTPPYA	NIc	NI
Phg2	ASLRTLTSLLPA	92 ± 10	NI
Phg3	NFMESLPRLGMH	NI	NI
Phg4d	Cyclo-CSWFEASYC	65 ± 7	90 ± 10
Phg5d	Cyclo-CLPTLHLLC	NI	NI
Phg6	AHLELRSNNMYF	NI	NI

^a Activity was analyzed in the in vitro reconstituted apoptosome.

^b Activity was analyzed in a cell extract-based assay.

^c NI, no inhibition found at the peptide concentration range analyzed.

^d Peptides Phg4 and Phg5, which contain two Cys residues in the N and the C terminus, were cycled (see “Experimental Procedures”).