FIGURE 6.

EGF does not reach FYVE-positive compartment in HeLa cells overexpressing PTP1B D/A. HeLa cells were seeded on coverslips and at 16 h postseeding transfected with cherry-PTP1B (Cherry-1B) WT (A) or D/A (B) and GFP-FYVE. 16 h post-transfection, cells were serum-starved in the presence of cycloheximide for 2 h and loaded with Alexa Fluor 647-EGF at 4 °C for 1 h. Cells were chased with warm medium without EGF. A, GFP-FYVE- and cherry-PTP1B WT-overexpressing cells. The merge of EGF (red) and FYVE (green) is shown. B, GFP-FYVE- and cherry-PTP1B D/A-overexpressing cells. The merge of EGF (red) and FYVE (green) is shown. Pearson's coefficient of co-localization, 0.07 ± 0.02 (0 min (0′)) and 0.44 ± 0.06 (30 min (30′)) for PTP1B WT-transfected cells and 0.05 ± 0.02 (0 min) and 0.18 ± 0.01 (30 min) for PTP1B D/A-transfected cells. p values, 0.4359 (0 min) and 0.0132 (30 min).