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. 2011 Nov 9;286(52):44542–44556. doi: 10.1074/jbc.M111.275073

FIGURE 5.

FIGURE 5.

PGAM5L is required for INrf2 and Bcl-xL interaction. A, Western analysis/immunoprecipitation. Hepa-1 cells were transfected with control or PGAM5 siRNA for 36 h, lysed, and immunoblotted (left). One mg of the same cell lysates from siRNA transfected cells was immunoprecipitated with anti-INrf2 antibody and immunoblotted with antibodies as shown. B, Western analysis/immunoprecipitation. Mutation of PGAM5-E79A/S80A abolished INrf2/Bcl-xL interaction. Hepa-1 cells were transfected with PGAM5L-V5 or mutant PGAM5L-E79A/S80A-V5 along with HA-INrf2 and FLAG-Bcl-xL plasmids and immunoblotted (top). Interactions of PGAM5L-V5 and mutant PGAM5L-E79A/S80A-V5 with HA-INrf2 and FLAG-Bcl-xL were analyzed by immunoprecipitation/immunoblotting (bottom panels). C, Western analysis/immunoprecipitation. Hepa-1 cells were transfected with control or Bcl-xL siRNA and immunoblotted with anti-Bcl-xL, anti-PGAM5L, and anti-INrf2 antibodies (left). One mg of lysates from transfected cells was immunoprecipitated with anti-INrf2 or anti-PGAM5L antibody and immunoblotted for Bcl-xL, PGAM5L, and INrf2.