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Size exclusion chromatography profile of HCNCB mutants. A, elution profile of HCN1CB R538E mutant, in the presence (red lines) and in the absence (black lines) of 1 mm cAMP. The profile of HCN1CB WT is shown for comparison as a dotted line. Shown are elution profiles of ΔC-linker HCNCB proteins (HCN1 (black), HCN2 (blue), and HCN4 (green)) in the absence (B) and in the presence (C) of 1 mm cAMP.
