TABLE 3.

Effect of mutations in the β4-β5 linker and in β-sheet 5 on the half-activation voltage of HCN1 (V_1/2), measured by two-electrode voltage clamp in intact oocytes

Channel	V1/2	n
HCN1 WT	−67.5 ± 0.13	11
G510S	−66.5 ± 0.25	4
S515G/S516N	−65.6 ± 0.23	5
G510S/S515G/S516N	−67.9 ± 0.34	5
M519T	−66.5 ± 0.65	3
R538E	−75.9 ± 0.35	4
HCN2 WT	−76.5 ± 0.04	7