FIGURE 3.

PhoP regulates expression of pks2 (A) and msl3 (B) in vivo in M. smegmatis. M. smegmatis strains harboring indicated constructs along with wild-type and mutant PhoP expression plasmids were grown in the absence or presence of ATc, and β-galactosidase activity from the transcription fusion was measured at indicated times. The values shown here are averages of at least three independent experiments. Insets compare expression of PhoP in crude extracts (24-h time point) containing equal amounts of total protein (as determined by Bradford assay) by Western blot using anti-PhoP antibody (Abexome Biosciences). As a loading control, the same crude extracts were probed with antibody against the β-subunit of RNA polymerase (Abcam).