TABLE 1.

Bacterial strains and plasmids used in this work

	Description	Source or Ref.
Strains
E. coli DH5α		Novagen
E. coli BL21(DE3)		56
M. tuberculosis H37Ra		ATCC25177
M. tuberculosis H37Rv		ATCC27294
M. smegmatis mc2155		57
Plasmids
pET-phoP	PhoP residues 1–247 cloned in pET15ba	34
pET-phoPD71N	Asp71 codon mutated to Asn in pET-phoP	17
pME1mL1	Mycobacterial protein expression vectorb	27
pME1mL1-phoP	PhoP residues 1–247 cloned in pME1mL1	This work
pME1mL1-phoPD71N	Asp71 mutated to Asn in pME1mL1-PhoP	This work
pSM128	Integrative promoter probe vector for mycobacteriac	26
pSM-pks2up1	pks2up1-lacZ fusion in pSM128	This work
pSM-pks2up1sDR2	Mutant pks2up1-lacZ fusion carrying changes in DR2 site	This work
pSM-msl3up1	msl3up1-lacZ fusion in pSM128	This work
pSM-msl3up1sDR2	Mutant msl3up1-lacZ fusion carrying changes in DR2 site	This work
pJEM15	Promoter-less E. coli-mycobacteria shuttle vectord	25
pJEM-phoP	Entire regulatory region along with phoP encoding gene (residues 1–247) cloned in pJEM15	This work
pJEM-phoPD71N	Asp71 codon of phoP mutated to Asn in pJEM-phoP	This work

^a Amp^r indicates ampicillin resistance.

^b Hyg^r indicates hygromycin resistance.

^c Str^r indicates streptomycin resistance.

^d Kan^r indicates kanamycin resistance.