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. 2011 Nov 9;286(52):45197–45208. doi: 10.1074/jbc.M111.307447

TABLE 1.

Bacterial strains and plasmids used in this work

Description Source or Ref.
Strains
    E. coli DH5α Novagen
    E. coli BL21(DE3) 56
    M. tuberculosis H37Ra ATCC25177
    M. tuberculosis H37Rv ATCC27294
    M. smegmatis mc2155 57

Plasmids
    pET-phoP PhoP residues 1–247 cloned in pET15ba 34
    pET-phoPD71N Asp71 codon mutated to Asn in pET-phoP 17
    pME1mL1 Mycobacterial protein expression vectorb 27
    pME1mL1-phoP PhoP residues 1–247 cloned in pME1mL1 This work
    pME1mL1-phoPD71N Asp71 mutated to Asn in pME1mL1-PhoP This work
    pSM128 Integrative promoter probe vector for mycobacteriac 26
    pSM-pks2up1 pks2up1-lacZ fusion in pSM128 This work
    pSM-pks2up1sDR2 Mutant pks2up1-lacZ fusion carrying changes in DR2 site This work
    pSM-msl3up1 msl3up1-lacZ fusion in pSM128 This work
    pSM-msl3up1sDR2 Mutant msl3up1-lacZ fusion carrying changes in DR2 site This work
    pJEM15 Promoter-less E. coli-mycobacteria shuttle vectord 25
    pJEM-phoP Entire regulatory region along with phoP encoding gene (residues 1–247) cloned in pJEM15 This work
    pJEM-phoPD71N Asp71 codon of phoP mutated to Asn in pJEM-phoP This work

a Ampr indicates ampicillin resistance.

b Hygr indicates hygromycin resistance.

c Strr indicates streptomycin resistance.

d Kanr indicates kanamycin resistance.