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. 2011 Nov 2;286(52):44433–44440. doi: 10.1074/jbc.M111.303891

FIGURE 1.

FIGURE 1.

Embryo-derived MCs. A, May Grünwald/Giemsa staining of WT, NDST1+/−, NDST1−/−, NDST2−/−, and NDST1+/−2−/− embryo-derived MCs. B, expression of MC proteases analyzed by Western blotting as described under “Experimental Procedures.” Two separate runs are shown. β-Actin expression served as a loading control. In the table below the blot, the level of expression of CPA3, MCPT5, and MCPT6 compared with β-actin is shown. C, quantitative PCR of NDST expression. Data are displayed as the -fold difference between expression of the respective NDST isoform in total embryo at day E11.5 (set at 1.0) and WT embryo-derived MCs and undifferentiated ES cells, respectively. C(t) values for the different samples were: NDST1, E11.5: 8.29, MCs: 9.32, ES cells: 10.73; NDST2, E11.5: 5.87, MCs: 3.68, ES cells: 8.10; NDST3, E11.5: 8.17, MCs: 7.35, ES cells: 13.26. NDST4, E11.5: 13.59. The experiment has been repeated with similar results.