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. 2011 Nov 15;286(52):44306–44318. doi: 10.1074/jbc.M111.282756

FIGURE 3.

FIGURE 3.

Identification of miR-469 target genes. A, panel of GRTH-targeted testicular genes derived from polysome microarray gene expression analysis (40) was selected for the study. miR-469 inhibits the expression of a luciferase construct containing coding region and 3′-UTR of protamine 2 (Prm2) and transition protein 2 (TP2) mRNA *, p < 0.01. No effect on testis-specific histone H1 gene (H1fnt), PGK2, Prm1, and TP1. B, recovery of miR-469 inhibition in Prm2 and TP2 by miR-469 inhibitor. Normalized luciferase activity (Renilla/Firefly) in NIH3T3 cells cotransfected with reporter constructs in the presence of scrambled RNA, miR-469 precursors, scrambled inhibitor, and or miR-469 inhibitor as indicated. Results are the means ± S.E. from three independent experiments with four culture replicates each. miR-469/scrambled inhibitor versus scrambled RNA/scrambled inhibitor in Prm2 and TP2 groups, *, p < 0.01 and miR-469/miR-469 inhibitor versus scrambled RNA/miR-469 inhibitor in Prm2 and TP2 groups; **, p > 0.05. Vector only indicates no differences among groups.