FIGURE 4.

Mapping of miR-469 response element(s) in protamine 2. Left panel, diagram of the constructs of mouse Prm2 full-length and deletions in the psi-CHECK2 vector. Coding region with deletion of ATG codon or 3′-UTR of Prm2 was subcloned in the downstream 3′-UTR of Renilla luciferase (Luc). Shaded bar indicates the coding region of Prm2, and solid square indicates two response elements of miR-469. Right panel, normalized luciferase activity (Renilla/Firefly) in NIH3T3 cells cotransfected with deletion constructs in the presence of scrambled RNA or miR-469 precursors, as indicated. Results are the means ± S.E. of three independent experiments performed in quadruplicate.