FIGURE 7.

miR-469 represses Prm2 and TP2 protein expression with minor effect on mRNA level. A, quantitative real time qPCR measurements of Prm2 and TP2 mRNA from transfected NIH3T3 cell line. Cells were cotransfected pCMV-FLAG-Prm2 or pCMV-FLAG-TP2 construct with either scrambled RNA (control, open bar) or miR-469 precursor (solid bar). B, Prm2 and TP2 protein expression level in the transfected NIH3T3 cells. Western blot analyses were performed using anti-FLAG antibody and anti-β-actin as an internal control. Results presented are from three independent studies. Data are presented as the means ± S.E.