TABLE 1.
Mass spectrometric parameters for amide-AGE, CML, and CEL quantification
| Retention time | Precursor ion |
Product ion 1a |
Product ion 2b |
Product ion 3b |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| m/z | DP | m/z | CE | CXP | m/z | CE | CXP | m/z | CE | CXP | ||
| min | amu | V | amu | eV | V | amu | eV | V | amu | eV | V | |
| N6-acetyl lysine | 11.7 | 189.2 | 30.0 | 126.1 | 18.0 | 10.0 | 84.2 | 31.0 | 5.0 | 143.1 | 14.0 | 10.0 |
| N6-formyl lysine | 8.2 | 175.1 | 25.0 | 112.1 | 20.0 | 13.0 | 84.1 | 35.0 | 7.0 | 129.1 | 15.0 | 13.0 |
| N6-lactoyl lysine | 12.1 | 219.2 | 32.0 | 156.2 | 20.0 | 8.0 | 84.1 | 35.0 | 9.0 | 173.1 | 17.0 | 8.0 |
| N6-glycerinyl lysine | 6.7 | 235.3 | 48.0 | 84.2 | 37.0 | 6.0 | 172.3 | 23.0 | 30.0 | 189.3 | 17.0 | 15.0 |
| CML | 6.9 | 205.1 | 50.0 | 130.1 | 17.0 | 9.5 | 84.1 | 46.0 | 13.0 | |||
| CEL | 11.5 | 219.1 | 54.0 | 130.1 | 18.0 | 11.0 | 84.1 | 33.0 | 7.00 | 173.0 | 18.0 | 15.0 |
a MRM transition used for quantification (quantifier).
b MRM transition used for confirmation (qualifier).