Skip to main content
. 2011 Nov 3;286(52):44491–44502. doi: 10.1074/jbc.M111.284463

FIGURE 5.

FIGURE 5.

Binding of HPA to H120N. A solution of H120N (40 μm)·FMNH− (16 μm) was mixed with air-saturated sodium phosphate buffer (pH 7.0) containing various concentrations of HPA: 0, 0.16, 0.4, 1, 2, 4, and 8 mm (concentrations after mixing). The kinetics was monitored at 390 and 446 nm. The traces at 390 and 446 nm from low to high HPA concentrations are indicated by the vertical and horizontal arrows, respectively. The inset is a plot of the absorbance change at 390 nm at a reaction time of 2 s versus the concentration of HPA. The data analysis in the inset indicates that HPA bound to C4a-hydroperoxy-FMN with a Kd value of 2.2 ± 0.7 mm.