TABLE 2.
Formation of the hydroxylated product (DHPA) from single-turnover reactions of His-120 and Ser-146 variants
DHPA product was not detected for the reactions of H120N·FMNH−, H120Q·FMNH−, H120Y·FMNH−, H120D·FMNH−, and H120E·FMNH− with oxygen in the presence of HPA (2 or 20 mm) using the HPLC method. The limit of detection was 5% (1.3 μm).
| pH | %DHPA |
|||
|---|---|---|---|---|
| H120K | H120R | S146A | S146C | |
| 6.0 | 76 ± 4 | 9 ± 1 | 72 ± 4 | 65 ± 6 |
| 6.5 | 80 ± 5 | 74 ± 1 | 59 ± 1 | |
| 7.0 | 81 ± 6 | 16 ± 1 | 73 ± 1 | 65 ± 2 |
| 7.5 | 78 ± 10 | 75 ± 8 | 65 ± 5 | |
| 8.0 | 80 ± 5 | 11 ± 1 | 76 ± 7 | 65 ± 4 |
| 8.5 | 84 ± 11 | 76 ± 3 | 64 ± 1 | |
| 9.0 | 72 ± 3 | 11 ± 1 | 76 ± 3 | 66 ± 1 |
| 9.5 | 71 ± 4 | 79 ± 8 | 47 ± 1 | |
| 10.0 | 70 ± 5 | 7 ± 1 | 78 ± 7 | 27 ± 4 |