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. 2011 Oct 20;286(52):44845–44854. doi: 10.1074/jbc.M111.225466

FIGURE 5.

FIGURE 5.

S100A7 overexpression reduces expression of downstream targets of the β-catenin/TCF4 pathway in ERα+ cells. A and B, 50 μg of cell lysates obtained from T47D/Vec, T47D/S100A7, MCF7/Vec, and MCF7/S100A7 cells were subjected to Western blotting using cyclin D1 (A) or c-myc (B) antibodies. C, tumors derived from mice injected with MCF7/Vec or MCF7/S100A7 cells were subjected to immunohistochemical staining for cyclin D1 or β-catenin. D, 50 μg of cell lysates were subjected to immunoblot analysis with TCF4 antibody or GAPDH. E, 50 μg of tumor lysates were subjected to Western blotting with TCF-4 antibody. GAPDH served as a loading control in all blots. All experiments were repeated three times, and a representative one is shown. The values below the blots show relative intensity obtained after densitometric analyses. *, p < 0.05 as calculated from Student's t test.