FIGURE 7.
TCF4 overexpression in S100A7-overexpressing cells restores the oncogenic properties. A, TCF4 was transiently transfected in MCF7/S100A7 in pcDNA3.1 vector using Lipofectamine according to the manufacturer's recommendations. 24 h after transfection, 5 × 103 cells were seeded in a 96-well tissue culture plate, and proliferation was measured using the MTT assay as described under “Experimental Procedures.” B, 50 μg of cell lysates of TCF4 and vector-transfected MCF7/S100A7 were subjected to Western blotting with anti-TCF4 or c-myc antibodies. GAPDH served as a loading control in all blots. The values below the blots show relative intensity obtained after densitometric analyses. *, p < 0.05 as calculated from one-way ANOVA. Error bars, S.D.