FIGURE 2.

Alterations of Lzts2 expression disrupt the convergence and extension movements and the formation of dorsoventral axis in zebrafish. A, embryos at one-cell stage were microinjected with 10 ng of lzts2-MO, 300 pg of lzts2 mRNA, or 10 ng of lzts2-MO plus 300 pg of lzts2 mRNA. Wild type embryos (WT) were used as the control. Lateral views at 6 hpf, animal pole toward the top and dorsal toward the right. Lateral and dorsal views at 12 hpf, anterior toward the top. B, total number of embryos examined (indicated at the bottom) and percentage of abnormal embryos in A. The phenotype of remaining embryos is similar to that of wild type. C, embryos at one-cell stage were injected with 10 ng of lzts2-MO or 300 pg of lzts2 mRNA. Morphological phenotypes of injected embryos at 48 hpf were imaged.