Table 1.
The stimulatory effect of EPS isolated from Lactobacillus rhamnosus KL37 and the whole bacterial cells on cytokine production by peritoneal macrophages
| TNF-α† | IL-6† | IL-10† | IL-12p40† | TNF-α vs IL-10‡ | IL-12p40‡ vs IL-10 | |
|---|---|---|---|---|---|---|
| Escherichia coli | 1413 ± 655** | 29,181 ± 5593*** | 857 ± 88*** | 3652 ± 847*** | 1.6:1 | 4:1 |
| LPS | 1085 ± 241** | 23,195 ± 1749*** | 104 ± 8*** | 1820 ± 328 ** | 10:1 | 17:1 |
| L. rhamnosus | 2107 ± 562*** | 15,659 ± 56*** | 1231 ± 396*** | 1006 ± 426*** | 1.7:1 | 0.8:1 |
| EPS | 469 ± 119* | 11,685 ± 2068*** | 77 ± 22* | 1243 ± 233** | 6:1 | 16:1 |
| Untreated | 167 ± 22 | 2073 ± 308 | 9 ± 4 | 385 ± 55 | 18:1 | 43:1 |
Cytokines were analysed by ELISA in supernatants collected from 24 h cultures of oil-induced peritoneal macrophages (5 × 105 per well) stimulated with exopolysaccharides (EPS) (100 μg/ml), Lipopolysaccharide (LPS) (1 μg/ml) or killed bacteria (107 cfu per well). Data are mean ± SEM values of three independent experiments.*P < 0.05, **P < 0.005, ***P < 0.001, treated vs. untreated macrophages.
†
Level of cytokines is expressed in pg/ml.
‡
The balance was calculated from data shown in this table.